Keratin Acidic (AE1) antibody (FITC)

Catalog No. ABIN5540046

The Mouse Monoclonal anti- antibody has been validated for IF, FACS and ICC. It is suitable to detect in samples from Human, Rat, Chicken, Cow, Mouse, Rabbit, Monkey and Pig.

Quick Overview for Keratin Acidic (AE1) antibody (FITC) (ABIN5540046)

Target: Keratin Acidic (AE1)

Reactivity: Human, Rat, Chicken, Cow, Mouse, Rabbit, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: AE1

Product Details

Specificity: This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.

Purification: Protein A Chromatography

Immunogen: Human epithelial keratin.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL (1/200-1/1,000) (Not tested in our lab).

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: acidic cytokeratin

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.