Actin antibody (N-Term)

Catalog No. ABIN5540760

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC (p), IHC (fro) and EIA. It is suitable to detect Actin in samples from Human, Rat, Rabbit and Pig.

Quick Overview for Actin antibody (N-Term) (ABIN5540760)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Rat, Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 3B3

Product Details anti-Actin Antibody

Binding Specificity: N-Term

Sequence: Ac-DEDETTALVC -COOH

Specificity: This 3B3 Monoclonal antibody is highly specific for Alpha-Skeletal Actin, and does not cross react with other Actin isoforms.

Purification: Purified

Immunogen: Peptide comprising the N-terminal nonapeptide of alpha-skeletal Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.

Isotype: IgG1

Application Details

Application Notes: ELISA. Flow Cytometry. Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: alpha skeletal muscle actin,acta1

Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development