CES1 antibody (HRP)

Catalog No. ABIN5540865

The Rabbit Polyclonal anti-CES1 antibody is suitable to detect CES1 in samples from Pig. It has been validated for WB and EIA.

Quick Overview for CES1 antibody (HRP) (ABIN5540865)

Target: CES1 (Carboxylesterase 1 (CES1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES1 antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CES1 Antibody (HRP)

Specificity: This antibody detects porcine esterase. Cross reactivity against esterase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Esterase [porcine liver].

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Esterase from porcine liver

Application Details

Application Notes: Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended Dilutions: Western blot : 1/1000-1/5000 Immunohistochemistry. ELISA : 1/14000. This product has been assayed against 1.0 μg of esterase [porcine liver] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Alternative Name: ces1,acat

UniProt: Q29550

Pathways: Monocarboxylic Acid Catabolic Process