Doublecortin antibody

Catalog No. ABIN5540930

This Chicken Polyclonal antibody specifically detects Doublecortin in IF, ICC and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for Doublecortin antibody (ABIN5540930)

Target: Doublecortin (DCX)

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Doublecortin Antibody

Purification: Affinity Chromatography

Immunogen: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Immunofluorescence/ Immnunocytochemistry. Immunohistochemistry. Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at a concentration of 2 μg/mL) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions : 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: doublecortin

UniProt: O43602