ASK1 antibody (AA 43-90)

Catalog No. ABIN5541032

This Rabbit Polyclonal antibody specifically detects ASK1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for ASK1 antibody (AA 43-90) (ABIN5541032)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ASK1 Antibody

Binding Specificity: AA 43-90

Specificity: This antibody detects endogenous levels of MEKK5 / ASK1 protein (region surrounding Thr77).

Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Immunogen: Synthetic peptide, corresponding to amino acids 43-90 of Human ASK1.

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 95 % pure by SDS-PAGE) Aff - Purified

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: mekk5,ask1

Background: Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response