CD55 antibody

Catalog No. ABIN5541170

This Rat Monoclonal antibody specifically detects CD55 in WB and FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD55 antibody (ABIN5541170)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 3D5

Product Details anti-CD55 Antibody

Specificity: The monoclonal antibody 3D5 recognizes complement decay accelerating factor (DAF), also designated as CD55.

Purification: Protein G Chromatography

Immunogen: NRK cells expressing transmembrane-anchored Mouse DAF

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. Western blot. The typical starting working dilution is 1/50.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS Preservatives: 0.02 % Sodium Azide Stabilizers: 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: cd55,daf

Background: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Mouse DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. DAF is broadly distributed among cells in contact with serum, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complementmediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.

UniProt: Q61475

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity