CD55 antibody

Catalog No. ABIN5541172

Product Details anti-CD55 Antibody

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: The monoclonal antibody RDII-24 recognizes complement decay accelerating factor (DAF), also designated as CD55.

Purification: Purified

Immunogen: NIH-3T3 cells expressing GPI-anchored Rat DAF

Clone: RDIII-24

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Ref.1,2). Western blot: Non-reduced with a band size of 60-70 kDa. As positive control CHO-Rat DAF hyper-expressing cells were used (Ref.1,2). Functional Studies: RDIII-7 antibody completely blocked the protective effect of expressed Rat DAF (Ref.2). Immuno Assays (Ref.3). Immunohistochemistry on Frozen Sections (Ref.2). The typical starting working dilution is 1/50.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Stabilizers: 0.1 % BSA

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: cd55,daf (CD55 Products)

Synonyms: CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody

Background: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement-mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.

UniProt: Q9QYJ9

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity