C-JUN antibody (AA 201-250)

Catalog No. ABIN5541526

The Rabbit Polyclonal anti-C-JUN antibody (ABIN5541526) specifically detects C-JUN in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for C-JUN antibody (AA 201-250) (ABIN5541526)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-C-JUN Antibody

Binding Specificity: AA 201-250

Specificity: The antibody detects endogenous levels of c-Jun protein (region surrounding Pro233). Species: Human, Mouse, Rat.

Purification: Affinity-chromatography using epitope-specific immunogen

Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human c-Jun.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western blot : 1/500-1/1000. Immunochemistry on Paraffin Sections : 1/50-1/200.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 95 % pure by SDS-PAGE) Aff - Purified

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: ap-1,c-jun

Background: Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA- response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins