LARGE antibody (AA 35-142)

Catalog No. ABIN5541530

This Mouse Monoclonal antibody specifically detects LARGE in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for LARGE antibody (AA 35-142) (ABIN5541530)

Target: LARGE (Like-Glycosyltransferase (LARGE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LARGE antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: LARGE-02

Product Details anti-LARGE Antibody

Binding Specificity: AA 35-142

Specificity: This antibody recognizes human LARGE1, a glycosyltransferase localizing mainly to the Golgi apparatus. Crossreactivity with LARGE2 was not determined.

Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)

Immunogen: Recombinant fragment of human LARGE1 (amino acids 35-142)

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry. Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 95 % pure by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS) Preservatives: 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LARGE

Target: LARGE (Like-Glycosyltransferase (LARGE))

Alternative Name: large

Background: LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.

UniProt: O95461