JAK2 antibody

Catalog No. ABIN5541875

This Rabbit Polyclonal antibody specifically detects JAK2 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for JAK2 antibody (ABIN5541875)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-JAK2 Antibody

Specificity: This antibody reacts with the 130 kDa JAK2 on human and other species with consensus sequence.

Purification: Protein G affinity

Immunogen: Synthetic peptide derived from the human JAK2 peptide, conjugated with KLH

Application Details

Application Notes: ELISA: 1/64,000 for detection of the JAK2 peptide coated on ELISA plate. Western blot: 1/2,000 to detect 130 kDa JAK2 in TF-1 cell extract.

Restrictions: For Research Use only

Handling

Buffer: 0.01M PBS, pH 7.4

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: tyrosine-protein kinase jak2

Background: Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that plays an important role in signal transduction through its association with receptors on the cell membrane. When these receptors are activated by extracellular signals, the receptors activate JAK2 which in turn phosphorylates the tyrosine residues on the receptors. The phosphorylation enables the binding of downstream signaling molecules, primarily, signal transducer and activator of transcription (STAT) proteins. STATs possess a SHdomain that specifically binds to phosphorylated tyrosine residue on the receptor. STAT in cytoplasma is activated after binding and migrates to nucleus to activate gene transcription. JAK2 mediates the transduction of a variety of extracellular signals in this pattern. The receptors that are associated with JAK2 include type II cytokine receptors, GM-CSF receptor family, gp130 receptor family, growth hormone receptor, prolactin receptor and EPO receptor.

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response