Luciferase antibody

Catalog No. ABIN5541890

This Rabbit Polyclonal antibody specifically detects Luciferase in WB, IF, DB, EIA and ICC. It exhibits reactivity toward Photinus pyralis.

Quick Overview for Luciferase antibody (ABIN5541890)

Target: Luciferase

Reactivity: Photinus pyralis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Product Details anti-Luciferase Antibody

Specificity: IgG fraction of Polyclonal Rabbit antiserum to Luciferase from Photinus pyralis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Immunogen: Luciferase isolated and purified from Photinus pyralis. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Working Dilutions for Non-precipitating antibody-binding techniques: 1/1000-1/10000. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.

Restrictions: For Research Use only

Handling

Buffer: Buffer System: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Luciferase

Target: Luciferase

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

UniProt: P08659