CELA1 antibody (Biotin)

Catalog No. ABIN5541903

The Rabbit Polyclonal anti-CELA1 antibody is suitable to detect CELA1 in samples from Pig. It has been validated for WB and EIA.

Quick Overview for CELA1 antibody (Biotin) (ABIN5541903)

Target: CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CELA1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CELA1 Antibody (Biotin)

Specificity: This antibody detects porcine elastase. Cross reactivity against elastase from other tissues and species has not been determined. Immunoelectrophoresis gives a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified elastase [porcine pancreas].

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Elastase from porcine pancreas

Application Details

Application Notes: Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: Western blot : 1/500-1/3,000. ELISA : 1/5,000-1/25,000 This product has been assayed against 1.0 μg of elastase [porcine pancreas] in a standard sandwich ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CELA1

Target: CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Alternative Name: elastase-1

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5 kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.

UniProt: P00772