CD4 antibody

Catalog No. ABIN5541941

This Mouse Monoclonal antibody specifically detects CD4 in FACS, IP and IHC (fro). It exhibits reactivity toward Horse. It has been mentioned in 2+ publications

Quick Overview for CD4 antibody (ABIN5541941)

Target: CD4

Reactivity: Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CVS4

Product Details anti-CD4 Antibody

Specificity: This anitbody reacts with T-helper cells, it recognises a 58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4. Species: Equine (Horse).

Purification: Affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Equine thymocytes

Isotype: IgG1

Application Details

Application Notes: Flow cytometry : 1/25-1/200, use 10 μL of this working dilution to label 10e6 cells in 100 μL. Immunohistochemistry on Frozen sections. Immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Containing 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CD4 antibody (ABIN5541941)

Go, Zhang, Timoney, Cook, Horohov, Balasuriya: "Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes." in: Journal of virology, Vol. 84, Issue 10, pp. 4898-911, (2010) (PubMed).

Goodman, Loregian, Perkins, Nugent, Buckles, Mercorelli, Kydd, Palù, Smith, Osterrieder, Davis-Poynter: "A point mutation in a herpesvirus polymerase determines neuropathogenicity." in: PLoS pathogens, Vol. 3, Issue 11, pp. e160, (2008) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: cd4

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

UniProt: F6Y6X8

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events