CD4 antibody (PE)

Catalog No. ABIN5541942

Product Details anti-CD4 Antibody (PE)

Target: CD4

Reactivity: Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This anitbody CVS4 recognizes Equine CD4, a 58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4. A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).

Purification: Affinity Chromatography on Protein G

Immunogen: Equine thymocytes. Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.

Clone: CVS4

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry : Use 10 μL of neat antibody to label 10 6 cells in 100 μL.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CD4 antibody (PE) (ABIN5541942)

De Schauwer, Piepers, Van de Walle, Demeyere, Hoogewijs, Govaere, Braeckmans, Van Soom, Meyer: "In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 81, Issue 4, pp. 312-23, (2012) (PubMed).

Go, Zhang, Timoney, Cook, Horohov, Balasuriya: "Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes." in: Journal of virology, Vol. 84, Issue 10, pp. 4898-911, (2010) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: cd4 (CD4 Products)

Synonyms: cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

UniProt: F6Y6X8

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events