PARP2 antibody (AA 233-583)

Catalog No. ABIN5541969

The Mouse Monoclonal anti-PARP2 antibody has been validated for WB, IF, ICC, FACS and EIA. It is suitable to detect PARP2 in samples from Human.

Quick Overview for PARP2 antibody (AA 233-583) (ABIN5541969)

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: AT29G4

Product Details anti-PARP2 Antibody

Binding Specificity: AA 233-583

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human PARP2 (233-583aa) purified from E. coli

Isotype: IgG2a

Application Details

Application Notes: ELISA. Flow Cytometry Immunocytochemistry/Immunofluorescence. Western blot / Immunoblot. The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:500.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PARP2

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Alternative Name: parp2

Background: PARP2 is poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

UniProt: Q9UGN5

Pathways: DNA Damage Repair