PARP2 antibody (Middle Region)

Catalog No. ABIN2778112

The Rabbit Polyclonal anti-PARP2 antibody (ABIN2778112) specifically detects PARP2 in WB and IF. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Horse, Rabbit and Guinea Pig samples.

Quick Overview for PARP2 antibody (Middle Region) (ABIN2778112)

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PARP2 Antibody

Binding Specificity: Middle Region

Purpose: PARP2 Antibody - middle region

Sequence: LLDLFEVEKD GEKEAFREDL HNRMLLWHGS RMSNWVGILS HGLRIAHPEA

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against PARP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARP2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108428

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PARP2

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Alternative Name: PARP2

Background:

Background Information: This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Gene Name: poly(ADP-ribose) polymerase 2

Alternative Symbols: ARTD2, ADPRT2, PARP-2, ADPRTL2, ADPRTL3, pADPRT-2

Protein Name: poly [ADP-ribose] polymerase 2

Molecular Weight: 66 kDa

Gene ID: 10038

NCBI Accession: NP_005475

UniProt: Q9UGN5

Pathways: DNA Damage Repair