MCM2 antibody

Catalog No. ABIN5542011

The Mouse Monoclonal anti-MCM2 antibody is suitable to detect MCM2 in samples from Human. It has been validated for WB, IF and ICC.

Quick Overview for MCM2 antibody (ABIN5542011)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 4B8

Product Details anti-MCM2 Antibody

Specificity: This antibody detects ~120 kDa of MCM2 protein from whole cell lysate of human cell lines.

Cross-Reactivity (Details): Does not react with Mouse (WR19L) and Rat (Rat1).

Purification: Protein A agarose

Immunogen: Recombinant human MCM2 protein corresponding to full-length amino acids

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL for chemiluminescence detection system. Immunocytochemistry: 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: mcm2

Background: In human cells, MCM4, - 6, and - 7 proteins form a stable complex, and MCM2 is loosely associated with this complex. The heterotrimeric MCM complex containing MCM4, - 6 and - 7 ha s DNA helicase activity, and such activity can be inhibited by the presence of MCM2. Cdc7/Dbf4 protein kinase, which is required for the initiation of DNA replication phosphorylates MCM2 in vitro and in vivo. From these observations, it's thought that phos phorylation of MCM2 may remove MCM2 from the MCM4, - 6, and - 7 complex to activate its DNA helicase activity at the onset of DNA replication.

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA