PPM1F antibody (AA 1-454)

Catalog No. ABIN5542151

This Mouse Monoclonal antibody specifically detects PPM1F in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for PPM1F antibody (AA 1-454) (ABIN5542151)

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPM1F antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT6D11

Product Details anti-PPM1F Antibody

Binding Specificity: AA 1-454

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human PPM1F (1-454aa) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PPM1F

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Alternative Name: protein phosphatase 1f,ppm1f

Background: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity