Cyclin D3 antibody

Catalog No. ABIN5557294

The Rabbit Monoclonal anti-Cyclin D3 antibody has been validated for WB and IF (cc). It is suitable to detect Cyclin D3 in samples from Human.

Quick Overview for Cyclin D3 antibody (ABIN5557294)

Target: Cyclin D3 (CCND3)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin D3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4A8

Product Details anti-Cyclin D3 Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant human Cyclin D3 protein, around C-terminal 200aa.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Alternative Name: Cyclin D3

Background:

Synonyms: G1/S-specific cyclin-D3, CCND3

Background: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Gene ID: 896

UniProt: P30281

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process