Cyclin E1 antibody (pThr77)

Catalog No. ABIN5557296

This Rabbit Monoclonal antibody specifically detects Cyclin E1 in WB and IF (cc). It exhibits reactivity toward Human.

Quick Overview for Cyclin E1 antibody (pThr77) (ABIN5557296)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 4G10

Product Details anti-Cyclin E1 Antibody

Binding Specificity: pThr77

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Synthetic peptide derived from human Cyclin E1(T77), around 50-100aa (phospho T77).

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background:

Synonyms: Cyclin E1 phospho T77, p-Cyclin E1 phospho T77, Cyclin E1 Thr77, CCNE 1, CCNE,CCNE1,Cyclin E1 antibody Cyclin Es,Cyclin Et,CyclinE, G1/S specic cyclin E.

Background: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases