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Recombinant Cyclin B2 antibody (N-Term)

Name: Recombinant Cyclin B2 antibody (N-Term)
SKU: ABIN5557369
Availability: InStock

The Rabbit Monoclonal anti-Cyclin B2 antibody is suitable to detect Cyclin B2 in samples from Human. It has been validated for WB, IF, ICC, IHC (p) and IHC (fro).

Lane 1: Hela, Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody at 1:1000 overnight at 4˚C. (Recombinant Cyclin B2 antibody (N-Term))

PC12 cells were stained with Cyclin B2 (2F4) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (Recombinant Cyclin B2 antibody (N-Term))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded mouse colon section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Recombinant Cyclin B2 antibody (N-Term))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse testis section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Recombinant Cyclin B2 antibody (N-Term))

IHC (p)

4 images

Catalog No. ABIN5557369

$490.77

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 8 to 12 Business Days

Quick Overview for Recombinant Cyclin B2 antibody (N-Term) (ABIN5557369)

Target

See all Cyclin B2 (CCNB2) Antibodies

Cyclin B2 (CCNB2)

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Cyclin B2 antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Binding Specificity
15

14

14

13

7

7

7

5

2

2

2

2

2

1

1

1

1

1

1

1
N-Term

Purpose

Cyclin B2 Recombinant Antibody

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

Recombinant human Cyclin B2 protein, around N-terminal 150aa.

Isotype

IgG
anti-Cyclin B2 (CCNB2) (AA 23-391) antibody
CCNB2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC, FACS Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 1-100) antibody
CCNB2 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 58-398) antibody
CCNB2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 7-163) antibody
CCNB2 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) antibody
KD Validated CCNB2 Reactivity: Human WB Host: Rabbit Monoclonal 25GB2920 unconjugated Recombinant Antibody

anti-Cyclin B2 (CCNB2) (pSer11) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42145 unconjugated

anti-Cyclin B2 (CCNB2) (pSer392) antibody
CCNB2 Reactivity: Mouse DB Host: Rabbit Polyclonal RB42081 unconjugated

anti-Cyclin B2 (CCNB2) (pSer22) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42147 unconjugated

anti-Cyclin B2 (CCNB2) (pSer10) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42143 unconjugated

anti-Cyclin B2 (CCNB2) (pSer392) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42149 unconjugated

Application Notes

WB(1:300-5000), IHC-P(1:50-200), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Cyclin B2 (CCNB2)

Alternative Name

Cyclin B2

Background

Synonyms: HsT17299, G2/mitotic-specific cyclin-B2, CCNB2
Background: Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Gene ID

9133

UniProt

O95067

Pathways

Cell Division Cycle, M Phase