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Cyclin B2 antibody

Name: Cyclin B2 antibody
SKU: ABIN5557369
Price: 490.77 USD
Availability: InStock

CCNB2 Reactivity: Human, Mouse, Rat WB, IF (cc), IHC (p) Host: Rabbit Monoclonal 2F4 unconjugated

Lane 1: Hela, Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody at 1:1000 overnight at 4˚C.

PC12 cells were stained with Cyclin B2 (2F4) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

IF (cc)

Paraformaldehyde-fixed, paraffin embedded mouse colon section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse testis section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

4 images

Catalog No. ABIN5557369

Datasheet as PDF

Target See all Cyclin B2 (CCNB2) Antibodies

Cyclin B2 (CCNB2)
Reactivity
87

30

13

4

4

4

4

3

2

2

2

2
Human, Mouse, Rat
Host
96

5

3
Rabbit
Compatible Secondaries
Clonality
89

15
Monoclonal
Conjugate
44

10

10

9

7

7

2

2

2

2

2

2

1

1

1

1

1
This Cyclin B2 antibody is un-conjugated
Application
56

49

42

13

13

9

6

5

5

4

3

2

1

1
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

Recombinant human Cyclin B2 protein, around N-terminal 150aa.

Clone

2F4

Isotype

IgG
anti-Cyclin B2 (CCNB2) (AA 1-100) antibody
CCNB2 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) antibody
CCNB2 Reactivity: Human WB, IHC, IF Host: Rabbit Monoclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 58-398) antibody
CCNB2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 7-163) antibody
CCNB2 Reactivity: Human ELISA, WB, IF Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (Internal Region) antibody
CCNB2 Reactivity: Human, Mouse, Rat ELISA, WB, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (pSer22) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42147 unconjugated

anti-Cyclin B2 (CCNB2) (pSer10) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42143 unconjugated

anti-Cyclin B2 (CCNB2) (pThr359) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42151 unconjugated

anti-Cyclin B2 (CCNB2) (pSer392) antibody
CCNB2 Reactivity: Human DB Host: Rabbit Polyclonal RB42149 unconjugated

anti-Cyclin B2 (CCNB2) (pSer392) antibody
CCNB2 Reactivity: Mouse DB Host: Rabbit Polyclonal RB42081 unconjugated

Application Notes

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Cyclin B2 (CCNB2)

Alternative Name

Cyclin B2 (CCNB2 Products)

Synonyms

HsT17299 antibody, CycB2 antibody, sb:cb303 antibody, cycB2 antibody, LOC100136691 antibody, C-B2 antibody, cyclinb2 antibody, hst17299 antibody, LOC100282653 antibody, cyclin B2 antibody, cyclin B2 S homeolog antibody, CCNB2 antibody, Ccnb2 antibody, ccnb2 antibody, LOC100136691 antibody, LOC100282653 antibody, ccnb2.S antibody

Background

Synonyms: HsT17299, G2/mitotic-specific cyclin-B2, CCNB2
Background: Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Gene ID

9133

UniProt

O95067

Pathways

Cell Division Cycle, M Phase