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Recombinant SMAD3 antibody (pSer423, pSer425)

Name: Recombinant SMAD3 antibody (pSer423, pSer425)
SKU: ABIN5557525
Availability: InStock

The Rabbit Monoclonal anti-SMAD3 antibody is suitable to detect SMAD3 in samples from Human. It has been validated for WB.

Lane 1: A431 cell lysates-untreated, Lane 2: A431 cell lysates-treated with TGF beta, probed with Smad3(S423/S425) (5C5 ) Monoclonal Antibody at 1:1000 overnight at 4˚C. (Recombinant SMAD3 antibody (pSer423, pSer425))

Paraformaldehyde-fixed, paraffin embedded mouse skin section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with Smad3(S423/S425) (5C5 ) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Recombinant SMAD3 antibody (pSer423, pSer425))

IHC (p)

A431 were fixed in paraformaldehyde, permeabilized with 0. (Recombinant SMAD3 antibody (pSer423, pSer425))

IF (cc)

3 images

Catalog No. ABIN5557525

$490.77

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 8 to 12 Business Days

Quick Overview for Recombinant SMAD3 antibody (pSer423, pSer425) (ABIN5557525)

Target

See all SMAD3 Antibodies

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This SMAD3 antibody is un-conjugated

Application

Western Blotting (WB)

Discover our top product SMAD3 Primary Antibody

Binding Specificity
53

47

29

29

27

12

12

11

8

6

6

6

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer423, pSer425

Purpose

Phospho-Smad3 (Ser423 + Ser425) Recombinant Antibody

Cross-Reactivity

Human

Predicted Reactivity

Mouse

Purification

Purified by Protein A.

Immunogen

KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425

Isotype

IgG
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 7F3 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody
SMAD3 Reactivity: Human WB, IHC, ELISA, FACS Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 Reactivity: Human WB, IHC, ELISA, IF, IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215) antibody
SMAD3 Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB24758 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
SMAD3 Reactivity: Human WB, IF, FACS, ICC, IHC (p), IHC (fro) Host: Rabbit Monoclonal unconjugated Recombinant Antibody

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215) antibody
SMAD3 Reactivity: Human, Mouse WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

Application Notes

WB(1:500-2000)

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name

Smad3

Background

Synonyms: Smad3(phospho Ser423/425), Smad3(phospho S423+S425), p-Smad3(Ser423+Ser425), hMAD 3, hSMAD3, HSPC193, JV15 2, JV152, MAD(mothers against decapentaplegic Drosophila) homolog 3, MAD3, MADH 3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD 3, SMAD, SMAD-3, SMAD3_HUMAN.

Gene ID

4088

UniProt

P84022

Pathways

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy