CRISPR-Cas9 (N-Term) antibody

Catalog No. ABIN5563965

The Mouse Monoclonal anti-CRISPR-Cas9 antibody is suitable to detect CRISPR-Cas9 in samples from Streptococcus pyogenes. It has been validated for WB, IF, IP and ChIP.

Quick Overview for CRISPR-Cas9 (N-Term) antibody (ABIN5563965)

Target: CRISPR-Cas9

Reactivity: Streptococcus pyogenes

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 8C1-F10

Product Details

Binding Specificity: N-Term

Purpose: Cas9 antibody (mAb) (Clone 8C1-F10)

Specificity: Mouse

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.

Isotype: IgG2b

Application Details

Application Notes: ChIP ICC/IF IP WB Validation was done using crude hybridoma supernatant. Individual optimization required. For Cas9, we also offer AbFlex Cas9 Recombinant Antibody (rAb). For details, see Catalog No. 91123.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details

Target: CRISPR-Cas9

Alternative Name: Cas9

Background: Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Molecular Weight: 160 kDa

NCBI Accession: WP_014407541