YY1 antibody (AA 249-414)

Catalog No. ABIN5563972

The Rabbit Polyclonal anti-YY1 antibody (ABIN5563972) specifically detects YY1 in WB, IHC, IP, ChIP-seq and CUT&Tag. The antibody is reactive with Human samples.

Quick Overview for YY1 antibody (AA 249-414) (ABIN5563972)

Target: YY1 (YY1 Transcription Factor (YY1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Product Details anti-YY1 Antibody

Binding Specificity: AA 249-414

Purpose: YY1 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.

Isotype: IgG

Application Details

Application Notes: ChIP-Seq: 10 µL per ChIP IP: 0.5 - 4 µL per IP WB*: 1:1,000 - 1:5,000 dilution IHC (FFPE): 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1

Background: YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).

Molecular Weight: 63 kDa

NCBI Accession: NP_003394