TET3 antibody (AA 976-1521)

Catalog No. ABIN5563988

This Rat Monoclonal antibody specifically detects TET3 in WB, IF, IP and ICC. It exhibits reactivity toward Mouse.It has been mentioned in 1 publication

Quick Overview for TET3 antibody (AA 976-1521) (ABIN5563988)

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TET3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 23B9

Product Details anti-TET3 Antibody

Binding Specificity: AA 976-1521

Purpose: Tet3 antibody (mAb) (Clone 23B9)

Specificity: Mouse

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding amino acids 976-1521 of mouse Tet3.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-TET3 antibody (ABIN5563988)

Bauer, Göbel, Nagaraj, Colantuoni, Wang, Müller, Kremmer, Rottach, Leonhardt: "Phosphorylation of TET proteins is regulated via O-GlcNAcylation by the O-linked N-acetylglucosamine transferase (OGT)." in: The Journal of biological chemistry, Vol. 290, Issue 8, pp. 4801-12, (2015) (PubMed).

Target Details for TET3

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Alternative Name: Tet3

Background: Tet3 (Tet Methylcytosine Dioxygenase 3) is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

Molecular Weight: 180 kDa

NCBI Accession: NP_898961

Pathways: Warburg Effect