Hexokinase 2 antibody (HRP)

Catalog No. ABIN5596731

Supplier Product No.: 200-4359-0100

The Rabbit Polyclonal anti-Hexokinase 2 antibody has been validated for WB and ELISA. It is suitable to detect Hexokinase 2 in samples from Saccharomyces cerevisiae.

Quick Overview for Hexokinase 2 antibody (HRP) (ABIN5596731)

Target: Hexokinase 2 (HK2)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-Hexokinase 2 Antibody (HRP)

Supplier Product No.: 200-4359-0100

Supplier: Rockland

Purpose: Hexokinase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].

Characteristics: Synonyms: rabbit anti-Hexokinase antibody HRP conjugation, peroxidase conjugation rabbit anti-Hexokinase antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB

Purification: Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Hexokinase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HEXOKINASE Peroxidase Conjugated Antibody has been tested by ELISA and western blot and can be used in applications such as Immunohistochemistry (IHC), and Immunofluorescence (IF). Specific conditions should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:1,000

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HXK2

Background: Background: HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.

Gene ID: 852639

UniProt: P04806

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect