HMW HCP antibody

Catalog No. ABIN5596742

Supplier Product No.: 200-401-j05

This Rabbit Polyclonal antibody specifically detects HMW HCP in ELISA and WB. It exhibits reactivity toward E. coli.

Quick Overview for HMW HCP antibody (ABIN5596742)

Target: HMW HCP (HMW Host Cell Proteins (HMW HCP))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMW HCP antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-HMW HCP Antibody

Supplier Product No.: 200-401-j05

Supplier: Rockland

Purpose: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.

Specificity: HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins.

Characteristics:

Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP

Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

Purification: HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Western Blot Dilution: 1:500 - 1:1000

ELISA Dilution: 1:1000-1:2000

Comment:

Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.38 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HMW HCP

Target: HMW HCP (HMW Host Cell Proteins (HMW HCP))

Alternative Name: HMW E.Coli Host Cell Proteins