LMW HCP antibody

Catalog No. ABIN5596745

Supplier Product No.: 200-401-j06

This Rabbit Polyclonal antibody specifically detects LMW HCP in ELISA, 2D-PAGE and WB. It exhibits reactivity toward E. coli.

Quick Overview for LMW HCP antibody (ABIN5596745)

Target: LMW HCP (LMW Host Cell Proteins (LMW HCP))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LMW HCP antibody is un-conjugated

Application: ELISA, Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)

Product Details anti-LMW HCP Antibody

Supplier Product No.: 200-401-j06

Supplier: Rockland

Purpose: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli.

Characteristics: Synonyms: rabbit anti-E.Coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-Low molecular Weight E.Coli Host Cell Proteins Antibody, Anti-Host Cell Protein, Low molecular weight E.Coli HCP proteins antibody

Purification: LMW Host Cell Protein A purified antibody is directed against low molecular weight E.coli Host Cell Proteins.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and has been tested by 2D-Page, western blotting, and ELISA.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:1000 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.29 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LMW HCP

Target: LMW HCP (LMW Host Cell Proteins (LMW HCP))

Alternative Name: LMW E.Coli Host Cell Protein

Background: Background: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli. This antibody has been analyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.