STAT5A antibody (2meArg31 (asymetric))

Catalog No. ABIN5596750

Supplier Product No.: 600-401-gh5

This anti-STAT5A antibody is a Rabbit Polyclonal antibody detecting STAT5A in WB, ELISA and DB. Suitable for Human.

Quick Overview for STAT5A antibody (2meArg31 (asymetric)) (ABIN5596750)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Product Details anti-STAT5A Antibody

Binding Specificity: 2meArg31 (asymetric)

Supplier Product No.: 600-401-gh5

Supplier: Rockland

Purpose: STAT5 R31-Me2a Antibody

Cross-Reactivity (Details): This antibody is specific for STAT5 R31/Me2a reacts with human STAT5.

Characteristics: Synonyms: rabbit anti-STAT5 Asym-dimethyl Arg31 antibody, rabbit anti-STAT5 R31me2a antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody

Purification: Anti-STAT5 R31/Me2a was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: STAT5A [Asym-dimethyl Arg31] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-STAT5 [Asym-dimethyl Arg31] antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500

ELISA Dilution: 1:5000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A

Background: Background: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5b. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.

UniProt: P42229

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation