IDO1 antibody (N-Term)
Quick Overview for IDO1 antibody (N-Term) (ABIN5596783)
Target
See all IDO1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Supplier Product No.
- 600-401-j44
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Supplier
- Rockland
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Purpose
- Ido Antibody
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Cross-Reactivity (Details)
- A BLAST analysis was used to suggest cross-reactivity with human, rat, and mouse based on 100 % sequence homology.
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Characteristics
- Synonyms: rabbit anti-Ido antibody, rabbit anti-Ido1 antibody, Indoleamine 2,3-dioxygenase 1, Ido-1, Ido1, Ido 1, Indoleamine-pyrrole 2,3-dioxygenase
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Purification
- Anti-IDO was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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Sterility
- Sterile filtered
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Immunogen
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Immunogen: IDO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse IDO.
Immunogen Type: Conjugated Peptide
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Isotype
- IgG
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Application Notes
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Application Note: Anti-IDO antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~41 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.05 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.05 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
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Alternative Name
- Ido1
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Background
- Background: Indoleamine 2, 3-dioxygenase (IDO) antibody initiates the first and rate limiting step of the kynurenine pathway. IDO has been shown to be important in inflammatory response and in the brain can be activated by interferon activated T cells. Anti-IDO antibody is ideal for investigators interested in Metabolism, Signal Transduction, and Cardiovascular research.
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Pathways
- Activated T Cell Proliferation
Target
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