Streptavidin antibody (Biotin)

Catalog No. ABIN5596791

Product Details anti-Streptavidin Antibody (Biotin)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to Biotin

Application: ELISA

Cross-Reactivity (Details): No reaction was observed against Avidin.

Purification: Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin.

Immunogen:

Immunogen: Streptavidin (Streptomyces avidinii)

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Biotin Conjugated Anti-Streptavidin Antibody has been assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentation in immunoassays should be performed by the researcher.

ELISA Dilution: 1:2,000-1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products

Background:

Synonyms: BAC, Streptavidin Biotin, Anti-Streptavidin BAC Antibody

Background: Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10-14 Mol/l to 10-15 Mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin thorught the antbody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interation.