BLM antibody (pSer186)

Catalog No. ABIN5596802

Supplier Product No.: 600-401-j61

This Rabbit Polyclonal antibody specifically detects BLM in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for BLM antibody (pSer186) (ABIN5596802)

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BLM Antibody

Binding Specificity: pSer186

Supplier Product No.: 600-401-j61

Supplier: Rockland

Purpose: BLM phospho S186 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-BLM pS186 Antibody, DNA helicase, RecQ-like type 2, RECQ2, RECQL3, Bloom syndrome protein

Purification: Anti-BLM pS186 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human S186 residue.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-BLM pS186 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-BLM pS186 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.66 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM

Background: Background: BLM pS186 product is a member of the RecQ helicase protein family. The protein contains 1417 amino acids, including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLS protein is known to associate and be phosphorylated by the ATR (ataxia telangiectasia, rad3+) protein, in response to genetic stress. The Bloom's syndrome gene product is thought to play a role in the etiology of two major human health problems, each of which is a very common complication of Bloom's syndrome, namely cancer and diabetes. Anti-BLM pS186 antibody is ideal for researchers interested in Epigenetics and Nuclear Signaling or DNA Damage & Repair research.

UniProt: P54132

Pathways: DNA Damage Repair