BLM antibody

Catalog No. ABIN7224220

The Rabbit Polyclonal anti-BLM antibody (ABIN7224220) specifically detects BLM in ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for BLM antibody (ABIN7224220)

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BLM Antibody

Purpose: Rabbit Anti-BLM Polyclonal Antibody

Specificity: BLM Polyclonal Antibody detects endogenous levels of BLM protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human BLM around the non-phosphorylation site of T99

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM

Background: BLM, RECQ2, RECQL3, Bloom syndrome protein, DNA helicase, RecQ-like type 2, RecQ2, RecQ protein-like 3The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination.

Gene ID: 641

UniProt: P54132

Pathways: DNA Damage Repair