CPY antibody (HRP)

Catalog No. ABIN5596850

Supplier Product No.: 200-403-135-0100

This Rabbit Polyclonal antibody specifically detects CPY in WB, ELISA and IHC. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for CPY antibody (HRP) (ABIN5596850)

Target: CPY (Carboxypeptidase Y (CPY))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPY antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CPY Antibody (HRP)

Supplier Product No.: 200-403-135-0100

Supplier: Rockland

Purpose: Carboxypeptidase Y Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker's Yeast].

Characteristics: Synonyms: rabbit anti-Carboxypeptidase Y antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

Purification: Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carboxypeptidase Y [Baker's Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Carboxypeptidase Y Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CPY

Target: CPY (Carboxypeptidase Y (CPY))

Alternative Name: Carboxypeptidase Y

Background: Background: Carboxypeptidase Y is an enzyme that catalyzes the reaction of the release of a C-terminal amino acid with broad specificity. Carboxypeptidase Y is a carboxypeptidase with optimum activity at pH 4.5-6.0. It is inhibited by diisopropyl fluorophosphate.

Gene ID: 855343

NCBI Accession: NP_014026

UniProt: P00729