beta Actin antibody

Catalog No. ABIN559692

Cited in 3+ publications. The Mouse Monoclonal anti-beta Actin antibody (Clone BA3R) (ABIN559692) specifically detects beta Actin in WB, ELISA, DB and ISt. The antibody is reactive with Human, Mouse, Rat, Chicken and Rabbit samples.

Quick Overview for beta Actin antibody (ABIN559692)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunostaining (ISt)

Clone: BA3R

Product Details anti-beta Actin Antibody

Specificity: Recognizes native and denatured forms of Beta-Actin (∼42kDa). Beta-Actin from human, mouse, rat, rabbit, and chicken / Beta-Actin from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: Beta-Actin N-terminal peptide-KLH conjugates

Isotype: IgG2b

Application Details

Application Notes: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-beta Actin antibody (ABIN559692)

Steponaitis, Kazlauskas, Skiriute, Valiulyte, Skauminas, Tamasauskas, Vaitkiene: "Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma." in: Oncology letters, Vol. 12, Issue 5, pp. 3305-3311, (2016) (PubMed).

Vaitkienė, Skiriutė, Steponaitis, Skauminas, Tamašauskas, Kazlauskas: "High level of Sema3C is associated with glioma malignancy." in: Diagnostic pathology, Vol. 10, pp. 58, (2015) (PubMed).

Skiriutė, Steponaitis, Vaitkienė, Mikučiūnas, Skauminas, Tamašauskas, Kazlauskas: "Glioma Malignancy-Dependent NDRG2 Gene Methylation and Downregulation Correlates with Poor Patient Outcome." in: Journal of Cancer, Vol. 5, Issue 6, pp. 446-56, (2014) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Beta-Actin

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction