JAK2 antibody (pTyr372)

Catalog No. ABIN5596931

Supplier Product No.: 600-401-j45

This Rabbit Polyclonal antibody specifically detects JAK2 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for JAK2 antibody (pTyr372) (ABIN5596931)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-JAK2 Antibody

Binding Specificity: pTyr372

Supplier Product No.: 600-401-j45

Supplier: Rockland

Purpose: JAK2 phospho Y372 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human, orangutan, rat, and pig based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-JAK2 pY372 antibody, JAK 2, JAK-2, Tyrosine-protein kinase JAK2, Janus kinase 2

Purification: Anti-JAK II was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: JAK II affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the pY372 region of human JAK II.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-JAK2 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~130 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background: Background: Janus Kinase 2 (JAK2) antibody is essential for signaling thorough a variety of cytokine receptors. Phosphorylation of JAK is essential for downstream signaling. JAK kinases are known to phosphorylate several substrate including members of the STAT family of proteins. Anti-JAK II antibody is ideal for researchers investigating Cancer, Signal Transduction, Epigenetics and Nuclear Signaling, and Cell Biology research.

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response