PAX6 antibody

Catalog No. ABIN5596956

Supplier Product No.: 200-301-r22

The Mouse Monoclonal anti-PAX6 antibody has been validated for WB, IF, ELISA and FM. It is suitable to detect PAX6 in samples from Human.

Quick Overview for PAX6 antibody (ABIN5596956)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Fluorescence Microscopy (FM)

Clone: 7H10-D3-D4-G5

Product Details anti-PAX6 Antibody

Supplier Product No.: 200-301-r22

Supplier: Rockland

Purpose: PAX6 Antibody

Cross-Reactivity (Details): Cross-reactivity with PAX6 from other sources has not been determined.

Characteristics: Synonyms: mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody

Purification: Anti-PAX6 was purified from clarified ascites by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:75,000 - 1:150,000

IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6

Background: Background: Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gene ID: 5080

NCBI Accession: NP_000271

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis