TET3 antibody (N-Term)

Catalog No. ABIN5596980

Supplier Product No.: 100-401-n94

The Rabbit Polyclonal anti-TET3 antibody has been validated for WB and ELISA. It is suitable to detect TET3 in samples from Human.

Quick Overview for TET3 antibody (N-Term) (ABIN5596980)

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TET3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TET3 Antibody

Binding Specificity: N-Term

Supplier Product No.: 100-401-n94

Supplier: Rockland

Purpose: TET3 Antibody

Characteristics: Synonyms: rabbit anti-TET3 Antibody, TET antibodies, Anti-TET Methylcytosine Dioxygenase 3, Anti-TET3 polyclonal antibody, KIAA0401 antibody, dioxygenase antibodies

Purification: Anti-TET3 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: Tet3 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180 kDa. This antibody is suitable for use by ELISA.

Western Blot Dilution: 1:5000

ELISA Dilution: 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TET3

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Alternative Name: TET3

Background: Background: Anti-Tet3 is expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Anti-Tet3 is involved in the epigenetic chromatin reprogramming of a zygote after its fertilization, and the demethylation occurs before the first cell division in the paternal pronucleus. Tet 3 antibody cannot bind to the maternal pronucleus chromatin due to DPPA3/PGC7 chromatin presence. Tet 3 functions as the dioxygenase which synthesizes a hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet3 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet3 plays an active role in catalyzing those conversions which have been suggested as the first step for cytosine DNA de-methylation. Tet 3 also recruits O-GlcNAc transferase OGT to aid H2B GlcNAcylation by OGT. Anti-TET3 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

Gene ID: 200424

NCBI Accession: NP_659430

UniProt: O43151

Pathways: Warburg Effect