SOD1 antibody (HRP)

Catalog No. ABIN5596995

Supplier Product No.: 200-4391-0100

The Rabbit Polyclonal anti-SOD1 antibody has been validated for WB and ELISA. It is suitable to detect SOD1 in samples from Cow.

Quick Overview for SOD1 antibody (HRP) (ABIN5596995)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-SOD1 Antibody (HRP)

Supplier Product No.: 200-4391-0100

Supplier: Rockland

Purpose: Superoxide Dismutase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].

Characteristics: Synonyms: rabbit anti-Superoxide Dismutase 1 Antibody HRP conjugation, peroxidase conjugated rabbit anti-SOD1 antibody, peroxidase conjugated rabbit anti-Superoxide Dismutase 1 Antibody, rabbit anti-SOD1 antibody HRP conjugation, ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody, SOD 1

Purification: Superoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Superoxide Dismutase [Bovine erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Superoxide Dismutase Peroxidase Conjugated Antibody has been tested by Western blot and is suitable to be assayed against 1.0 μg of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background: Background: Superoxide Dismutase destroys radicals which are normally produced within the cells and which are toxic to biological systems. They are an important antioxidant defense in nearly all cells exposed to oxygen. Mutations in the Superoxide Dismutase enzyme can cause ALS, A4V, G93A, Down Syndrome, ARDS, and COPD.

Gene ID: 281495

UniProt: P00442

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis