GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody

Details for Product No. ABIN561018, Supplier: Log in to see
Antigen
  • DYT14
  • DYT5
  • DYT5a
  • GCH
  • GTP-CH-1
  • GTPCH1
  • HPABH4B
  • GTP-CH
  • GTPCH
  • Gch
  • MGC89622
  • gch1a
  • wu:fc16b10
  • zgc:195269
  • GTP cyclohydrolase 1
  • GTP cyclohydrolase 1-like
  • GCH1
  • Gch1
  • gch1
  • Weevi_1190
  • Halhy_0838
  • Sinme_1220
  • Sphch_1077
  • LOC100136794
Epitope
AA 84-172, partial
15
11
10
9
8
7
5
2
2
2
2
1
1
1
1
Reactivity
Human
83
32
31
5
5
4
3
3
2
2
2
2
2
1
1
1
Host
Mouse
57
17
11
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
6
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
69
48
27
13
7
6
6
1
1
Options
Supplier
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Supplier Product No.
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Immunogen GCH1 (NP_000152, 84 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: ENPQRQGLLK TPWRAASAMQ FFTKGYQETI SDVLNDAIFD EDHDEMVIVK DIDMFSMCEH HLVPFVGKVH IGYLPNKQVL GLSKLARIV
Clone 4A12
Isotype IgG2a kappa
Cross-Reactivity Human
Alternative Name GCH1 (GCH1 Antibody Abstract)
Background Full Gene Name: GTP cyclohydrolase 1
Synonyms: DYT14,DYT5,GCH,GTP-CH-1,GTPCH1
Gene ID 2643
NCBI Accession NP_000152, NM_000161
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Supplier Images
Immunostaining (ISt) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody (ABIN561018) Immunoperoxidase of monoclonal antibody to GCH1 on formalin-fixed paraffin-embedded h...
Western Blotting (WB) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody (ABIN561018) GCH1 monoclonal antibody (M01), clone 4A12 Western Blot analysis of GCH1 expression i...
ELISA image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody (ABIN561018) Detection limit for recombinant GST tagged GCH1 is approximately 0.3ng/ml as a captur...
Immunoprecipitation (IP) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody (ABIN561018) Immunoprecipitation of GCH1 transfected lysate using anti-GCH1 monoclonal antibody an...
Western Blotting (WB) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody (ABIN561018) Western Blot analysis of GCH1 expression in transfected 293T cell line by GCH1 monocl...
 image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-172), (partial) antibody (ABIN561018) Western Blot detection against Immunogen (35.53 KDa) .
Product cited in: Jabs, Oelze, Mikhed, Stamm, Kröller-Schön, Welschof, Jansen, Hausding, Kopp, Steven, Schulz, Stasch, Münzel, Daiber: "Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats." in: Vascular pharmacology, Vol. 71, pp. 181-91, 2015 (PubMed).

Barajas-Espinosa, Basye, Jesse, Yan, Quan, Chen: "Redox activation of DUSP4 by N-acetylcysteine protects endothelial cells from Cd²?-induced apoptosis." in: Free radical biology & medicine, Vol. 74, pp. 188-99, 2014 (PubMed).

Kossmann, Hu, Steven, Schönfelder, Fraccarollo, Mikhed, Brähler, Knorr, Brandt, Karbach, Becker, Oelze, Bauersachs, Widder, Münzel, Daiber, Wenzel: "Inflammatory monocytes determine endothelial nitric-oxide synthase uncoupling and nitro-oxidative stress induced by angiotensin II." in: The Journal of biological chemistry, Vol. 289, Issue 40, pp. 27540-50, 2014 (PubMed).

Pickert, Lim, Weigert, Häussler, Myrczek, Waldner, Labocha, Ferreirós, Geisslinger, Lötsch, Becker, Brüne, Tegeder: "Inhibition of GTP cyclohydrolase attenuates tumor growth by reducing angiogenesis and M2-like polarization of tumor associated macrophages." in: International journal of cancer. Journal international du cancer, Vol. 132, Issue 3, pp. 591-604, 2012 (PubMed).

Oelze, Knorr, Schuhmacher, Heeren, Otto, Schulz, Reifenberg, Wenzel, Münzel, Daiber: "Vascular dysfunction in streptozotocin-induced experimental diabetes strictly depends on insulin deficiency." in: Journal of vascular research, Vol. 48, Issue 4, pp. 275-84, 2011 (PubMed).

Teng, Du, Xu, Bakhutashvili, Eis, Shi, Pritchard, Konduri: "Sepiapterin improves angiogenesis of pulmonary artery endothelial cells with in utero pulmonary hypertension by recoupling endothelial nitric oxide synthase." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 301, Issue 3, pp. L334-45, 2011 (PubMed).

Schuhmacher, Oelze, Bollmann, Kleinert, Otto, Heeren, Steven, Hausding, Knorr, Pautz, Reifenberg, Schulz, Gori, Wenzel, Münzel, Daiber: "Vascular dysfunction in experimental diabetes is improved by pentaerithrityl tetranitrate but not isosorbide-5-mononitrate therapy." in: Diabetes, Vol. 60, Issue 10, pp. 2608-16, 2011 (PubMed).

Kröller-Schön, Schulz, Wenzel, Kleschyov, Hortmann, Torzewski, Oelze, Renné, Daiber, Münzel: "Differential effects of heart rate reduction with ivabradine in two models of endothelial dysfunction and oxidative stress." in: Basic research in cardiology, Vol. 106, Issue 6, pp. 1147-58, 2011 (PubMed).

Wenzel, Daiber, Oelze, Brandt, Closs, Xu, Thum, Bauersachs, Ertl, Zou, Förstermann, Münzel: "Mechanisms underlying recoupling of eNOS by HMG-CoA reductase inhibition in a rat model of streptozotocin-induced diabetes mellitus." in: Atherosclerosis, Vol. 198, Issue 1, pp. 65-76, 2008 (PubMed).

Seujange, Eiam-Ong, Tirawatnapong, Eiam-Ong: "Role of angiotensin II on dihydrofolate reductase, GTP-cyclohydrolase 1 and nitric oxide synthase expressions in renal ischemia-reperfusion." in: American journal of nephrology, Vol. 28, Issue 4, pp. 692-700, 2008 (PubMed).

Widder, Chen, Li, Dikalov, Thöny, Hatakeyama, Harrison: "Regulation of tetrahydrobiopterin biosynthesis by shear stress." in: Circulation research, Vol. 101, Issue 8, pp. 830-8, 2007 (PubMed).

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