SMAD3 antibody (AA 1-110)

Catalog No. ABIN561717

Cited in 3+ publications. The Mouse Monoclonal anti-SMAD3 antibody (Clone 4D5) (ABIN561717) specifically detects SMAD3 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for SMAD3 antibody (AA 1-110) (ABIN561717)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4D5

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 1-110

Purpose: Mouse monoclonal antibody raised against a partial recombinant SMAD3.

Sequence: MSSILPFTPP IVKRLLGWKK GEQNGQEEKW CEKAVKSLVK KLKKTGQLDE LEKAITTQNV NTKCITIPRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELRAMELCE

Cross-Reactivity: Human, Rat

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SMAD3 (NP_005893, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SMAD3 antibody (ABIN561717)

Jiang, Huang, Huang, Chen, Jhong, Chang, Chen, Chang, Chen, Lee, Chen, Yeh, Matsuura: "Dysregulation of the TGFBI gene is involved in the oncogenic activity of the nonsense mutation of hepatitis B virus surface gene sW182*." in: Biochimica et biophysica acta, Vol. 1842, Issue 7, pp. 1080-7, (2014) (PubMed).

Lamba, Wang, Tran, Ouspenskaia, Libasci, Hébert, Miller, Bernard: "Activin A regulates porcine follicle-stimulating hormone beta-subunit transcription via cooperative actions of SMADs and FOXL2." in: Endocrinology, Vol. 151, Issue 11, pp. 5456-67, (2010) (PubMed).

Morita, Mayanagi, Sobue: "Dual roles of myocardin-related transcription factors in epithelial mesenchymal transition via slug induction and actin remodeling." in: The Journal of cell biology, Vol. 179, Issue 5, pp. 1027-42, (2007) (PubMed).

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: Full Gene Name: SMAD family member 3
Synonyms: DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396

Gene ID: 4088

NCBI Accession: NM_005902

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy