Pleiotrophin antibody (AA 45-154)

Catalog No. ABIN562528

This Mouse Monoclonal antibody specifically detects Pleiotrophin in WB, ELISA and IHC (p). It exhibits reactivity toward Human. It has been mentioned in 8+ publications

Quick Overview for Pleiotrophin antibody (AA 45-154) (ABIN562528)

Target: Pleiotrophin (PTN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Pleiotrophin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C3

Product Details anti-Pleiotrophin Antibody

Binding Specificity: AA 45-154

Purpose: Mouse monoclonal antibody raised against a partial recombinant PTN.

Sequence: SDCGEWQWSV CVPTSGDCGL GTREGTRTGA ECKQTMKTQR CKIPCNWKKQ FGAECKYQFQ AWGECDLNTA LKTRTGSLKR ALHNAECQKT VTISKPCGKL TKPKPQAESK

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PTN (NP_002816, 45 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Pleiotrophin antibody (ABIN562528)

He, Boulant, Stanifer, Guo, Nießen, Chen, Felix, Bergmann, Strobel, Schimmack: "The link between menin and pleiotrophin in the tumor biology of pancreatic neuroendocrine neoplasms." in: Cancer science, Vol. 113, Issue 5, pp. 1575-1586, (2022) (PubMed).

Koutsioumpa, Polytarchou, Courty, Zhang, Kieffer, Mikelis, Skandalis, Hellman, Iliopoulos, Papadimitriou: "Interplay between ?v?3 integrin and nucleolin regulates human endothelial and glioma cell migration." in: The Journal of biological chemistry, Vol. 288, Issue 1, pp. 343-54, (2013) (PubMed).

Kaspiris, Mikelis, Heroult, Khaldi, Grivas, Kouvaras, Dangas, Vasiliadis, Lioté, Courty, Papadimitriou: "Expression of the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the serum, cartilage and subchondral bone of patients with osteoarthritis." in: Joint, bone, spine : revue du rhumatisme, Vol. 80, Issue 4, pp. 407-13, (2013) (PubMed).

Miao, Ding, Zhang, Xiao, Qi, Luo, Di, Tao, Fang: "Pleiotrophin promotes microglia proliferation and secretion of neurotrophic factors by activating extracellular signal-regulated kinase 1/2 pathway." in: Neuroscience research, Vol. 74, Issue 3-4, pp. 269-76, (2012) (PubMed).

Tsirmoula, Dimas, Hatziapostolou, Lamprou, Ravazoula, Papadimitriou: "Implications of pleiotrophin in human PC3 prostate cancer cell growth in vivo." in: Cancer science, Vol. 103, Issue 10, pp. 1826-32, (2012) (PubMed).

Mikelis, Lamprou, Koutsioumpa, Koutsioubas, Spyranti, Zompra, Spiliopoulos, Vradis, Katsoris, Spyroulias, Cordopatis, Courty, Papadimitriou: "A peptide corresponding to the C-terminal region of pleiotrophin inhibits angiogenesis in vivo and in vitro." in: Journal of cellular biochemistry, Vol. 112, Issue 6, pp. 1532-43, (2011) (PubMed).

Koutsioumpa, Hatziapostolou, Mikelis, Koolwijk, Papadimitriou: "Aprotinin stimulates angiogenesis and human endothelial cell migration through the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta." in: European journal of pharmacology, Vol. 602, Issue 2-3, pp. 245-9, (2009) (PubMed).

Mikelis, Sfaelou, Koutsioumpa, Kieffer, Papadimitriou: "Integrin alpha(v)beta(3) is a pleiotrophin receptor required for pleiotrophin-induced endothelial cell migration through receptor protein tyrosine phosphatase beta/zeta." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 5, pp. 1459-69, (2009) (PubMed).

Target Details for Pleiotrophin

Target: Pleiotrophin (PTN)

Alternative Name: PTN

Background: Full Gene Name: pleiotrophin
Synonyms: HARP,HBGF8,HBNF,NEGF1

Gene ID: 5764

NCBI Accession: NM_002825

Pathways: RTK Signaling