PCNA antibody

Catalog No. ABIN5633264

The Mouse Monoclonal anti-PCNA antibody (Clone PC10) (ABIN5633264) specifically detects PCNA in WB, FACS, IP, IHC (p), ICC and IHC (fro). The antibody is reactive with Human, Mouse, Rat, Chicken, Drosophila melanogaster and Non-Human Primate samples.

Quick Overview for PCNA antibody (ABIN5633264)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Drosophila melanogaster, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PC10

Product Details anti-PCNA Antibody

Purpose: Anti-PCNA Purified

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse, Rat, Chicken, Drosophila

Characteristics: The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: recombinant rat PCNA

Isotype: IgG2a, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: Proliferating cell nuclear antigen,PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.,ATLD2

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy