PCNA antibody
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- Target See all PCNA Antibodies
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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Reactivity
- Human, Mouse, Rat, Chicken, Drosophila melanogaster, Non-Human Primate
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PCNA antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.
- Cross-Reactivity (Details)
- Human, Non-Human Primates, Mouse, Rat, Chicken, Drosophila
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- recombinant rat PCNA
- Clone
- PC10
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product PCNA Primary Antibody
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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibodyPCNA Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17486 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibodyPCNA Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 7H4F8 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA Reactivity: Human WB, IHC, IF, IHC (p), IP, ICC, IHC (fro), FISH Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 1G7 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA Reactivity: Human WB, ELISA Host: Mouse Monoclonal S1 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibodyPCNA Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibodyPCNA Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Rhesus Monkey WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1H10 unconjugated
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- Application Notes
- Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Alternative Name
- PCNA (PCNA Products)
- Background
- Proliferating cell nuclear antigen,PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.,ATLD2
- Gene ID
- 5111
- UniProt
- P12004
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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