CD11b antibody

Catalog No. ABIN5633268

This Rat Monoclonal antibody specifically detects CD11b in FACS, IP and IHC (fro). It exhibits reactivity toward Human, Mouse, Rabbit and Non-Human Primate.

Quick Overview for CD11b antibody (ABIN5633268)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit, Non-Human Primate

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M1-70

Product Details anti-CD11b Antibody

Purpose: Anti-Ms CD11b Purified

Specificity: The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse, Rabbit

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: B10 mouse spleen cells enriched for T cells

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Gene ID: 16409

UniProt: G5E8F1

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation