TYMS antibody (AA 60-174)

Catalog No. ABIN5646952

The Mouse Monoclonal anti-TYMS antibody has been validated for IHC (p). It is suitable to detect TYMS in samples from Human.

Quick Overview for TYMS antibody (AA 60-174) (ABIN5646952)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TYMS-1884

Product Details anti-TYMS Antibody

Binding Specificity: AA 60-174

Characteristics: This antibody recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS, TYMS). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: A recombinant human parital protein corresponding to amino acids 60-174 was used as the immunogen for this TYMS antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: The optimal dilution of the TYMS antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the TYMS antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase

Background: This antibody recognizes a protein of 36  kDa, identified as Thymidylate Synthase (TS, TYMS). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Pathways: Mitotic G1-G1/S Phases