DYKDDDDK Tag antibody (Atto 390)
Quick Overview for DYKDDDDK Tag antibody (Atto 390) (ABIN5651028)
Target
Host
Clonality
Conjugate
Application
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Specificity
- Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
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Purification
- Peptide Affinity Purified
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Immunogen
- Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
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Application Notes
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
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Comment
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A 1:1000 dilution of ABIN5651028 was sufficient for detection of DYKDDDDK Tag in 15 μg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Conjugated antibodies should be stored at 4°C
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- DYKDDDDK Tag
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Target Type
- Tag
Target
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