RACGAP1 antibody (AA 1-110)

Catalog No. ABIN565414

This Mouse Monoclonal antibody specifically detects RACGAP1 in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for RACGAP1 antibody (AA 1-110) (ABIN565414)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1G6

Product Details anti-RACGAP1 Antibody

Binding Specificity: AA 1-110

Purpose: Mouse monoclonal antibody raised against a partial recombinant RACGAP1.

Sequence: MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE LGKYKDLLMK AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI QLIREMLMCD

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: RACGAP1 (AAH32754, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-RACGAP1 antibody (ABIN565414)

Lekomtsev, Su, Pye, Blight, Sundaramoorthy, Takaki, Collinson, Cherepanov, Divecha, Petronczki: "Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis." in: Nature, Vol. 492, Issue 7428, pp. 276-9, (2012) (PubMed).

Wang, Ooi, Hui: "Upregulation of Rac GTPase-activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 18, pp. 6040-51, (2011) (PubMed).

Wolfe, Takaki, Petronczki, Glotzer: "Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation." in: PLoS biology, Vol. 7, Issue 5, pp. e1000110, (2009) (PubMed).

Burkard, Maciejowski, Rodriguez-Bravo, Repka, Lowery, Clauser, Zhang, Shokat, Carr, Yaffe, Jallepalli: "Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells." in: PLoS biology, Vol. 7, Issue 5, pp. e1000111, (2009) (PubMed).

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: RACGAP1

Background: Full Gene Name: Rac GTPase activating protein 1
Synonyms: HsCYK-4,ID-GAP,MgcRacGAP

Gene ID: 29127

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor