K-RAS antibody (AA 100-189) (APC)

Catalog No. ABIN5667957

The Rabbit Polyclonal anti-K-RAS antibody has been validated for WB. It is suitable to detect K-RAS in samples from Rat.

Quick Overview for K-RAS antibody (AA 100-189) (APC) (ABIN5667957)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is conjugated to APC

Application: Western Blotting (WB)

Product Details anti-K-RAS Antibody (APC)

Binding Specificity: AA 100-189

Specificity: Detects ~29 kDa. Band is higher than the predicted molecular weight due to post translational modifications.

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the C-terminal of Human KRAS (aa. 100-189)

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of SPC-776 was sufficient for detection of HRAS in 15 μg of cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Gene ID: 3845

NCBI Accession: NP_004976

UniProt: P01116