Recombinant CD35 antibody

Catalog No. ABIN5668015

The Rabbit Chimeric anti-CD35 antibody is suitable to detect CD35 in samples from Mouse. It has been validated for FACS, WB, IP and IHC (fro).

Quick Overview for Recombinant CD35 antibody (ABIN5668015)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 8C12

Product Details anti-CD35 Antibody

Purpose: Anti-CD35 (complement receptor 1) [8C12], Rabbit IgG, kappa

Specificity:

Recognises an epitope on CD35 (CR1, 190 kDa), but not on CD21 (CR2, 145-150 kDa), which are alternative splice variants of primary Cr2 gene transcripts.

Application Notes original Clone: This antibody recognises murine CD35, expressed on the majority of peripheral B-cells and peritoneal macrophages and upon fMet-Leu-Phe stimulation also on

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: Purified murine CR1 protein.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Rat

OriginalFormat: IgG2a

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35 (complement receptor 1)

Background: CR1 , C3BR, C4BR, CD35, KN

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity